Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne
Abstract
Rotala rotundifolia (Buch-Ham. ex Roxb.) Koehne is a medicinal aquatic plant belonging to the family Lythraceae. Due to its ability to accumulate heavy metals, it also has a potential to be used for wastewater treatment. In this study, The effects of the combinations of 0.05-0.25 mg/L Gibberellic acid (GA3) and 0.25 mg/L Thidiazuron (TDZ) on in vitro callus formation from the full leaf, upper half of leaf and lower half of leaf explants of R. rotundifolia were investigated. The highest percentage of callus formation (100%) from the full leaf, upper leaf and lower leaf explants were obtained on MS medium containing 0,25 mg/L GA3 and 0,25 mg/L TDZ, 0.05 and 0.25 mg/L GA3 + 0,25 mg/L TDZ and 0,05 mg/L GA3 + 0,25 mg/L TDZ. More callus concentrations were recorded in culture media containing GA3 at low concentrations. The shortest callus formation time was determined on the lower leaf explant cultured on MS medium containing 0.05 and 0.25 mg/L GA3 + 0.25 mg/L TDZ and on the 10th day. Various secondary metabolites from callus of R. rotundifolia using plant tissue culture techniques can be obtained and these metabolites can be used in the pharmaceutical field. In addition, callus formations can help gene transfer studies.
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