Antibacterial and antifungal effects of the leaf, seed, seed coat and fruit capsule of Aesculus hippocastanum (Sapindaceae) extracts



The present study describes the antimicrobial activity of the leaf, seed, seed coat and fruit capsule of horse-chestnut (Aesculus hippocastanum, L.) extracts from the Ordu Province of Turkey. These extracts were first prepared with ethanol solvent. The antimicrobial activity of the extracts was then assessed using the disc diffusion method against 7 bacteria and 1 yeast. The antimicrobial test results revealed that each the crude extracts of A. hippocastanum exhibited very weakly possess antibacterial activity in the case of both Gram-positive and Gram-negative bacteria. The most sensitive microorganism to A. hippocastanum samples were Proteus vulgaris and Listeria monocytogenes. The results presented in this paper suggest the possibility that A. hippocastanum possesses a new antimicrobial compound that has a weakly effect against some Gram-negative and Gram-positive bacteria, but did not against fungi.


Sensitive microorganisms, Proteus vulgaris, Listeria monocytogenes.

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Anitha R., Geetha R.V., Lakshmi T. 2011. In vitro evaluation of antibacterial activity of aqueous and ethanolic extracts of Aesculus hippocastanum on oral microbes. Asian Journal of Pharmaceutical and Clinical Research, 4(4): 90­92.

Baytop T. 1984. Health treatment in Turkey Using plant extracts, Istanbul Univ., No. 3255, Sanal matbaacılık, Istanbul, Turkey.

Bisset N.G. 1994. Herbal drugs and phytopharmaceuticals - a handbook for practice on a scientific basis. Medpharm Scientific Publishers, Stuttgart.

Bombardelli E., Morazzoni P., Griffini A. 1996. Aesculus hippocastanum L. Fitoterapia, 67: 483-511.

Christenhusz M.J.M., Byng J.W. 2016. The number of known plants species in the world and its annual increase. Phytotaxa, 261(3): 201-217.

Davis P.H. 1966-1988. Flora of Turkey and the East Aegean Islands. Edinburgh University Press, Edinburgh.

Duke J.A. 1985. CRC handbook of medicinal herbs. Boca Raton: CRC Press.

Duke J.A. 1985. CRC handbook of medicinal herbs. Boca Raton: CRC Press.

Goodman G.A., Rall T.W., Nies A.S., Taylor P. 1991. Las Bases Farmacologicas de la Terapeutica 8th ed., Editorial M.dica Panamericana, Mexico DF.

Harborne J.B., Baxter H. 1993. Phytochemical dictionary. a handbook of bioactive compounds from plants. Taylor and Francis, London.

Holopainen M., Jabordar L., Kauppien V., Seppanen-Laukso I. 1988. Antimicrobial Activity of Some Finnish Ericaceous plants. Acta Pharmaceutica Fennica, 97(4): 197-202.

Lau A.F., Siedlecki J., Anleitner J., Patterson G.M.L., Caplan F.R., Moore R.E. 1993. Inhibition of reverse transcriptase activity by extracts of cultured blue-green algae (Cyanophyta). Planta Medica, 59:148-151.

Newall C.A., Anderson L.A., Phillipson J.D. 1996. Herbal Medicines. A guide for health-care professionals. The Pharmaceutical Press, London.

Parekh J., Chanda S.V. 2008. Antibacterial activity of aqueous and alcoholic extracts of 34 indian medicinal plants against some Staphylococcus Species. Turkish Journal of Biology, 32: 63-71.

Peirce A. 1999. The american pharmaceutical association practical guide to natural medicines. New York: William Morrow and Company, Inc.

Robbers J.E., Tyler V.E. 1999. Tyler's herbs of choice: The therapeutic use of phytomedicinals. New York: Haworth Herbal Press.

Ronald M.A. 1990. Microbiologia, Compania Editorial Continental S.A. de C.V., Mexico, D.F.

Taylor J.L.S., Rabe T., McGraw L.J. 2001. Towards the scientific validation of traditional medicinal plants. Plant Growth Regulation, 34(1): 23-37.

WHO. 2002-2005. Traditional Medicine Strategy, Geneva: World Health Organization.

Wilkinson J.A., Brown A.M. 1999. Horse chestnut-Aesculus hippocastanum: potential applications in cosmetic skin-care products. International Journal of Cosmetics Science, 21(6): 437-447.

Zhang X. 2004. Traditional medicine: its importance and protection. In: Protecting and Promoting Traditional Knowledge: Systems, National Experiences and International Dimensions. Part 1. The Role of Traditional Knowledge in Healthcare and Agriculture-Twarog S, Kapoor P, eds. New York: United Nations.

Zinhener H., Mear W.K. 1972. Biology of Antibiotics. Springer-Verlag, New York.


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